Background: Gotu Kola (Centella asiatica) has been used as a traditional medicine for many years to cure differentkinds of diseases. Studies have been reported that Gotu Kola extracts might be used as a cure for oral diseases suchas periodontal disease. In the present study, Gotu Kola leaves extracted with water will be used to evaluate its effecton some microorganisms living in the human saliva using minimum inhibitory concentration (MIC) method.Material and Method:Gotu Kola fresh leaves extract have been used with water as a solvent, a rotary evaporatorwas used to separate the solvent from the extract. The following microorganisms: Streptococci, Lactobacilli, andStaphylococcus aureus have been isolated fromthe Saliva of ten volunteers participated in the present study.Nutrient broth tubes have been prepared for MIC test, where various concentrations of the Gotu Kola extracts(0.5mg/ml, 1 mg/ml, 2 mg/ml, 3 mg/ml, 4 mg/ml, and 5 mg/ml) were added, respectively. The tubes incubated at370C for 48h.Results:The MIC test shows that a concentration of 4 mg/ml have the ability to inhibit the growth of oral Lactobacilliand 8 mg/ml has the ability to inhibit the growth of S. aureuswhich may be due to asiaticoside and asiatic acidwhich are active ingredients that the leaves extracts consists of. There was no MIC for Streptococci.Conclusion:The Gotu Kola leaves extract can be used to inhibit the growth of some oral microorganisms at certainconcentration.