Non-alcoholic fatty liver disease (NAFLD) includes a broad spectrum of fat-induced liverinjury, ranging from mild steatosis to cirrhosis and liver failure. This study investigates thehepatoprotective properties of garlic and onion in NAFLD rat model. Ninety male Sprague-Dawley rats were randomly divided into 9 groups; normal (I), NAFLD induced with high fat diet(HFD; II), NAFLD switched to regular diet (RD; III), NAFLD-HFD or NAFLD-RD treated withgarlic (IV, V), onion (VI, VII) or the combined garlic+onion (VIII, IX) respectively. A NAFLDrat model was established by feeding the animals with a high-fat diet for 12 wk. These animalswere then treated with garlic or/and onion or vehicle for 8 wk (weeks 13-20) and then killed toobtain serum samples and liver tissues. Liver histology, lipids, parameters of oxidative stress,TNF-α and TGF-β were measured. The liver in NAFLD-HFD showed typical steatosis,accompanied with mild to moderate lobular inflammatory cell infiltration. Serum levels of ALT,AST, ALP, leptin, cholesterol, triglycerides, TNF-α, TGF-β and hepatic MDA were significantlyincreased (P<0.05) compared with normal group. This was accompanied with reduction of hepaticGSH, GR, GPx, GST, SOD and serum adiponectin. These changes were to a less degree inNAFLD-RD group. Combined administration of garlic+onion produced a better and significantdecrease in liver steatosis, serum liver enzymes, oxidative markers and lipid peroxidation versuseach one alone. In the same time, NAFLD-induced inflammation was also mitigated via reductionof TNF-α and TGF-β. In addition, these results were better in the group IX versus group VIII.