Objective: The aim of this study was to screen patients with orallesions for the presence of Human Papilloma Virus (HPV) types16 and 18.Methods: Sixty patients aged between 11-80 years with a mean ageof 46 years were examined using immunohistological techniques.All samples were retrieved from RICK during the period fromAugust 2009 to August 2010. Out of 60 patients, 50 had OralSquamous Cell Carcinomas (OSCCs) and the remaining ten hadbenign oral lesions, included as internal control.Results: Of the 50 patients with OSCCs, 10 (20%) showedpositive immunohistochemical results for HPV types 16 and18 of which 50% were detected among males and 50% weredemonstrated among females. The ten positive findings wereImmunophenotyped as follows: five were positive with HPVtype 16, four with type 18 and one was positive for HPV types16 and18. All patients with benign oral lesions were negative forHPV immunohistochemistry.Conclusion: The study suggests the role of HPV 16 and 18 in theetiology of oral cancers in different parts of Sudan. However, theuse of molecular techniques such as PCR are needed to confirmthe results of immunohistochemistry in the role of the HPV indeveloping of OSCC in Sudan.