This study identified the zoonotic endo-parasites and ecto-parasites of Rattus rattus. A total of 41 rats of house (black) rat and Norway (brown) rat were trapped from two regions of Gaza strip. After dissection, isolated protozoa, nematodes and cestodes were identified respectively according standard keys. The results showed that prevalence of intestinal parasites among rats was 24/41 (58.5%) and males were infected more than females. A high prevalence of protozoa was in autumn compared to other seasons. The intestinal parasites were encountered: G. lamblia 6 (14.6%); E. histolytica/dispar 7 (17.1%); Isospora 4 (9.8%); Acanthocephala 1 (2.4%); Syphacia obvelata 6 (20%); Heligmonoides josephi 3(10%); Strongyloides egg 1 (2.4%); Hymenolepis diminuta 15 (36.6%). The insects were Xenopsylla cheopis 7 (17.1%); Polyplax spinulosa 3 (7.3%).