Objectives: Vitamin D deficiency is a global health problem, its role as an immune modulator has been recently emphasized.The evidence is increasingly pointing towards vitamin D significant role in reducing the incidence of autoimmune diseases.However, at this time the research on its role in autoimmune and thyroid disease is not conclusive.We aimed to examine the relationship between hypothyroidism and vitamin D deficiency and to clarify the relation betweenserum calcium levels with hypothyroid disease. Subjects and Methods: Serum vitamin D (25-OH) levels were measured in 30 patients with hypothyroidism and 30 healthysubjects, utilizing the spectrophotometric method. Vitamin D deficiency was designated at levels lower than 20 ng/ml. Thyroidhormones (TSH, T3 and T4) and calcium levels were evaluated in all participants. Results: Serum 25(OH) vit D was significantly lower in hypothyroid patients than in controls (t=-11.128, P =0.000). Its level wasinsignificantly decreased in females than male patients (t=- 1.32, P >0.05). Moreover, serum calcium levels recorded asignificant decrease in hypothyroid patients when compared to controls (t= -5.69, P = 0.000). Conclusion: Our results indicated that patients with hypothyroidism suffered from hypovitaminosis D with hypocalcaemia that issignificantly associated with the degree and severity of the hypothyroidism. That encourages the advisability of vit Dsupplementation and recommends the screening for Vitamin D deficiency and serum calcium levels for all hypothyroid patients.