Biosurfactants are surface-active compounds synthesized by a wide array of microorganisms. They are “amphipathic” molecules having both hydrophobic and hydrophilic domains. These are capable of lowering the surfacetension and the interfacial tension of the growthmedium. Biosurfactants have different chemicalstructures; lipopeptides, glycolipids, neutral lipids and fatty acids. They are non-toxic biomolecules that are biodegradable. Biosurfactants also exhibit strongemulsification of hydrophobic compounds and formstable emulsions. The low water solubility of these hydrophobic compounds confines their accessibility to microorganisms, which is a potential problem forbioremediation of contaminated sites. Microbiallyproduced surfactants enhance the bioavailability of these hydrophobic compounds for bioremediation. Therefore, biosurfactant-enhanced solubility of pollutants haspotential applications in bioremediation. Not only are the biosurfactants useful in a variety of industrialprocesses, they are also of vital importance to the microbes in adhesion, emulsification, bioavailability,desorption and defence strategy.