This study aimed to the discovery of new biologically active natural products with hormonelikeactivity from Butea frondosa Roxb., Family Leguminosae growing in Egypt. The investigation of estrogenlikeactivity showed that both the successive non-polar and methanolic extracts showed a significantestrogen-like activity in immature female rats. GLC analysis the non-polar bioactive revealed the presence ofeicosane (22.5%) and β-amyrin (20.5%) as the major components in the unsaponifiable matter, while palmitic(24.9%) and linoleic (36.8%) acids were the main saturated and unsaturated fatty acids. The successive bioactivemethanolic extract was subjected to chromatographic separation to yield five flavonoids identified asvicenin II, vitexin, chrysoeriol 7-o-β-D-glucuronic acid, 6,8-di-C-rhamnosyl apigenin and luteolin.